International Standard
ISO/TS 21569-5:2016
Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 5: Real-time PCR based screening method for the detection of the FMV promoter (P-FMV) DNA sequence
Reference number
ISO/TS 21569-5:2016
Edición 1
2016-11
International Standard
Vista previa
ISO/TS 21569-5:2016
69340
No disponible en español
Publicado (Edición 1, 2016)
Esta norma se revisó y confirmó por última vez en 2023. Por lo tanto, esta versión es la actual.

ISO/TS 21569-5:2016

ISO/TS 21569-5:2016
69340
Idioma
Formato
CHF 63
Convertir Franco suizo (CHF) a tu moneda

Resumen

ISO/TS 21569-5:2016 specifies a procedure for the detection of a DNA sequence used in genetically modified (GM) plants by means of a real-time PCR (polymerase chain reaction). The method detects a 78 base pairs long segment of the Figwort mosaic virus 34S promoter DNA sequence. This segment in some GM plants is indicated as FMV promoter (P-FMV) and in other GM plants as FMV enhancer (E-FMV).

The method was developed and validated for the analysis of DNA extracted from foodstuffs. It may be suitable also for analysis of other products such as feedstuffs and seeds. The procedure requires the extraction of an adequate quantity and quality of amplifiable DNA from the test sample.

The DNA sequence amplified by the P-FMV element-specific method can be detected in samples which contain DNA of the naturally occurring Figwort mosaic virus. For this reason, it is necessary to confirm a positive screening result. Further analyses are required using construct-specific or event specific methods.

Informaciones generales

  •  : Publicado
     : 2016-11
    : Norma Internacional confirmada [90.93]
  •  : 1
     : 10
  • ISO/TC 34/SC 16
    67.050 
  • RSS actualizaciones

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