ISO/PRF TS 21569-7
u
ISO/PRF TS 21569-7
84434

Abstract 

This proposed Technical Specification describes a procedure for the detection of a DNA sequence of the open reading frame V (ORF V) from Cauliflower Mosaic Virus (CaMV) and a procedure for the detection of the DNA sequence of the nopalin synthase (nos) gene from tumor-inducing (Ti) plasmids of phytopathogenic Rhizobium radiobacter (formerly named Agrobacterium tumefaciens). The procedures can be used in the context of screening for genetically modified plants to further clarify a positive PCR result for a specific promoter and/or terminator of CaMV (P-35S, T-35S) and of the nos gene (P-nos, T-nos), respectively. This clarification is important, if no known genetically modified plant event could be identified and the sample may contain natural CaMV and/or Rhizobium radiobacter Ti plasmid derived DNA. Both methods are based on real-time PCR and are applicable for the analysis of DNA extracted from foodstuffs and other products such as feedstuffs and seeds/grains. The application of the methods requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix. With appropriate calibration material, the CaMV ORF V and/or nos copy number can be estimated and compared with the estimated copy number for the promoter (P-35S, P-nos) and/or the terminator (T-35S, T-nos) sequences, respectively. Thereby, conclusions are possible about the presence of an unknown GMO in addition to any detected CaMV and/or Rhizobium radiobacter Ti plasmid DNA in a test sample.


General information 

  •  :  Under development
  •  : 1
  •  : ISO/TC 34/SC 16 Horizontal methods for molecular biomarker analysis
  •  :
    67.050 General methods of tests and analysis for food products

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