Reference number
ISO 15216-1:2017
© ISO 2025
International Standard
ISO 15216-1:2017
Microbiology of the food chain — Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR — Part 1: Method for quantification
Edition 1
2017-03
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Published (Edition 1, 2017)
This publication was last reviewed and confirmed in 2022. Therefore this version remains current.

ISO 15216-1:2017

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Format
CHF 177
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Abstract

ISO 15216-1:2017 specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.

This method is not validated for detection of the target viruses in other foodstuffs (including multi-component foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, food surfaces or other matrices.

General information

  • Status
     : Published
    Publication date
     : 2017-03
    Stage
    : International Standard confirmed [90.93]
  • Edition
     : 1
    Number of pages
     : 48
  • Technical Committee :
    ISO/TC 34/SC 9
    ICS :
    07.100.30 
  • RSS updates

 Amendments

Amendments are issued when it is found that new material may need to be added to an existing standardization document. They may also include editorial or technical corrections to be applied to the existing document.

Amendment 1

Edition 2021

Language
Format
CHF 18
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