ISO 21527-1:2008 specifies a horizontal method for the enumeration of viable yeasts and moulds in products, intended for human consumption or feeding of animals, having a water activity greater than 0,95 [eggs, meat, dairy products (except milk powder), fruits, vegetables, fresh pastes, etc.], by means of the colony count technique at 25 °C plus or minus 1 °C.
ISO 21527-1:2008 does not allow the enumeration of mould spores. Neither the identification of fungal flora nor the examination of foods for mycotoxins lie within the scope of ISO 21527-1:2008. The method specified in ISO 21527-1:2008 is not suitable for enumeration of heat-resistant fungi, such as Byssochlamys fulva or Byssochlamys nivea, in canned or bottled fruit and vegetables.