ISO 21115:2019

недоступно на русском языке

Опубликовано (Версия 1, 2019)

ISO 21115:2019

CHF 173

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Тезис

This document specifies a method for the determination of fish acute toxicity using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. Cells in confluent monolayers in 24-well tissue culture plates are exposed to water samples, such as surface waters or different kinds of effluents, or to chemicals for 24 h and, thereafter, cell viability is assessed based on fluorescent cell viability indicator dyes (see 4.1). Data are then expressed as a percentage of unexposed control and toxicity quantified based on the percentage of cell viability versus the percentage of effluent or the chemical concentration in response curves (see Clause 9).

Общая информация

Текущий статус
: Опубликовано

Дата публикации
: 2019-04

Этап
: Рассылка краткого отчета о пересмотре [90.60]
Версия
: 1
Технический комитет :
ISO/TC 147/SC 5

ICS :
13.060.70
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